Prof.Huangxian Ju

Issuing time:2021-09-18 22:07

Professor Ju huangxian.jpg

Name:

Huangxian Ju

Title:

Professor

Affiliation:

State Key Laboratory of   Analytical Chemistry for Life Science, Nanjing University

Email:

hxju@nju.edu.cn

100-word   biography:

Director of State Key Laboratory of   Analytical Chemistry for Life Science (Nanjing University). Fellows of   International Society of Electrochemistry and Royal Society of Chemistry. B.S.,   M.S. and Ph.D degrees from Nanjing University. Won the National Funds for National   Distinguished Young Scholars (2003), Changjiang Professor (2007), Chief Scientist   of “973” Program (2009). Research interests: analytical biochemistry,   nanobiosensing, bioimaging and molecular diagnosis. 713 SCI papers (501/648   papers in IF>5/3 journals). Authored 29 patents, 6 English books, 7   Chinese books and 20 chapters for books. 36177 citations (34848 by other   authors), h-index: 98 in SCI journals (Google Scholar h-index 107, > 41000   citations).

Title:

Theranostics of Cancer with Gene Recognition

Abstract:

Gene recognition has extensively been applied in   bioanalysis and bioimaging, whether in vitro or in vivo, as well as   biomedicine such as clinical diagnosis and disease therapy due to its   excellent specificity, which is of significance in the design of signal   switch or signal amplifier for highly sensitive biosensing and cell-specific bioimaging of different biomolecules, and the development of siRNA delivery systems and drug   vectors for controllable   intracellular siRNA or drug release and precise therapy of cancer.The rational functionalization of nanomaterials with target-specific   DNA moieties to recognize different receptors both on cell surface and in   cells has led to a large number of versatile theranostic nanosystems. Here I   report several theranostic nanosystems developed in my group based on the   specific gene recognition. Firstly, several signal switches were designed for   sensitive bioimaging and in situ detection of cancer-related biomolecules   [1-6]. By functionalizing nanoparticles with cell-targeting moity and conjugated gene probe, a target-cell-specific   delivery strategy for imaging and detection of intracellular miRNA was   developed [1]. By interval hybridization of modified hairpin DNA probe pairs,   a responsive “nano string light” was   proposed for highly efficient mRNA imaging in living cells [2]. A photo zipper locked DNA   nanomachine was also assembled for precise miRNA imaging in living cells [3].   Besides RNA, two switchable fluorescent probes were developed for in situ   “off-on” imaging of intracellular   telomerase by its catalysis toward synthesis of telomeric repeats [4,5], and a hierarchical coding   strategy was proposed for live cell imaging of protein-specific glycoform   [6]. Secondly, some siRNA delivery systems   were designed for gene therapy of cancer [7-10]. A DNA dual lock-and-key   strategy was designed for cell-subtype-specific siRNA delivery [7], two   upconversion nanoprobes were developed for near-infrared modulated efficient   siRNA delivery and therapy [8,9], and in situ siRNA assembly was achieved in   living cells for gene therapy [10]. Thirdly, to enhance therapeutic   efficiency, a   DNA-azobenzene nanopump was designed for controllable intracellular drug   release [11], a near-infrared   photo-switched microRNA amplifier was proposed for precise photodynamic   therapy of early-stage cancers [12], and a DNA   nanomachine via computation across cancer cell membrane was recently developed   for precise therapy of solid tumor [13].

References (Some of the publications can be downloaded from   https://cms.nju.edu.cn/hxju/lwlz/ )

1. H.F. Dong, J.P. Lei, H.X. Ju, F. Zhi, H. Wang, W.J. G, Z. Zhu, F.   Yan, Angew. Chem. Int. Ed. 2012, 51, 4607.

2. K.W. Ren, Y.F. Xu, Y. Liu, M. Yang, H.X. Ju,   via accelerated DNA cascade reaction,ACS Nano. 2018, 12, 263.

3. Y. Zhang, Y.   Zhang, X.B. Zhang, Y.Y. Li, Y.L. He, Y. Liu, H.X. Ju, Chem. Sci. 2020, 11,   6289-6296.

4. R.C. Qian, L.   Ding, H.X. Ju, J. Am. Chem. Soc.2013, 135, 13282.

5. R.C. Qian, L. Ding, L. Yan, M. Lin, H.X. Ju, J. Am. Chem. Soc. 2014, 136, 8205.

6. S.Q.   Li, Y.R. Liu, L. Liu, Y.M. F, L. Ding, H.X. Ju, Angew. Chem. Int. Ed. 2018,   57, 12007.

7. K.W. Ren, Y. Liu,   J. Wu, Y. Zhang, J. Zhu, M. Yang, H.   . Ju*, Nat. Commun. 2016, 7, 13580.

8. Y. Zhang, K.W.   Ren, X.B. Zhang, Z.C. Chao, Y.Q. Yang, D.J. Ye, Z.H. Dai, Y. Liu, H.X. Ju, Biomaterials2018, 163, 55.

9. Y.L. He, S.W.   Guo, L.N. Wu, P.W. Chen, L.Y. Wang, Y. Liu, H.X. Ju, Biomaterials 2019,   225, 119501.

10. K.W. Ren, Y.   Zhang, X.B. Zhang, Y. Liu, M. Yang, H.X. Ju, ACS Nano2018, 12,   10797.

11. Y.   Zhang, Y. Zhang, G. B. Song, Y.L. He, X.B. Zhang, Y. Liu, H.X. Ju, Angew. Chem. Int. Ed.2019, 58, 18207.

12. Y.   Zhang, W.W. Chen, Y. Zhang, X.B. Zhang, Y. Liu, H.X. Ju, Angew. Chem. Int. Ed.2020,   59, 21454.

13.Y. Zhang, W.W. Chen, X.B.   Zhang, Y.L. He, Y. Liu*, H.X. Ju, J. Am. Chem. Soc.2021, 10.1021/jacs.1c06361,published: on Sept.   12.