State Key Laboratory of bioelectronics, School of biological Science and Medical engineering, Southeast University
Email:
xwzhao@seu.edu.cn
100-word biography:
Xiangwei Zhao is a professor of State Key Laboratory of Bioelectrtonics, Southeast University. His research focus on biomedical detection and analysis based on micro/nanotechnology, including biophotonics, spatial transcriptomics, brain organoids, POCT, etc. He has published more than 70 peer reviewed papers, 20 issued patents and won the First Prize of Natural Science of the Ministry of Education and Gold Medal of the Geneva International Invention Exhibition.
Title:
Microscopic sampling based spatial transcriptomics at single cell scale
Abstract:
Transcriptome reflects which genes are actively being up- and downregulated. With the development of single-cell RNA sequencing, it became possible to measure transcriptomes at a single-cell resolution. However, the spatial position of the cell in the tissue is also a very important trait that associated with the development and cell types, which single cell RNA sequencing could not provide. Therefore, spatial transcriptomics (ST), which could tell “who” and “where”, attract more and more attentions. Although there are kinds of ST techniques, many challenges still remain to be addressedMicrodialysis, among which the data yield per position is of great importance. More data means much more information and cost-effeciency. In this presentation, we would like to introduce techniques of microscopic sampling of tissue samples with spatial informations in low and middle throughput means and the subsequent sequencing pipeline for high transcriptomic data yield, as well as the future of high content ST.