Prof. Hiroyuki Noji


Hiroyuki Noji




The University of Tokyo


100-word biography:

Since I proved rotary catalysis of F1 ATPase motor   protein (Nature 1997), I have been pursuing the elucidation of   chemomechanical coupling mechanism of F1-ATPase (Cell   1998, Nature 2001, 2004, 2005, PNAS 2020a, 2020b,   Science 2011, Nat. Comm. 2021). I have also established   digital bioassay by use of femtoliter reactor array device (Nature   biotechnology 2005, Lab on a chip 2012, Analytical   chemistry 2017). This technology has been applied for artificial cell   reactor project (Scientific Advances 2019, ACS Nano   2020) with aim to create autonomous artificial cell systems in bottom-up   approach (ACS Syn. Bio. 2021).


Digitization of   Bioassays


We developed   femto-liter reactor array device (FRAD) that display over million water-in-oil   droplets with size of femto-liter range. One can readily encapsulate bio- or   non-biological molecules into the FRAD reactors, by spreading assay mixture   on the device and sealing the reactors with oil or air. The extremely small   volume features of the reactors on FRAD allows very sensitive bioassay at   single-molecule level referred as to ‘digital bioassay’. The massive number   of the reactor enables to identify highly active but very rare functional   molecules among a large numbers of library. Several examples will be   introduced including Digital ELISA, Digital Influenza Virus Counting, and   single-transporter analysis. The high biocompatibility of FRAD reactors also   allows the reconstitution of molecular systems such as cell-free gene   expression and replication. In the last part of the presentation, I will   introduce the concept of FRAD in the context of synthetic biology, and show   digital gene expression with an application for the engineering of alkaline   phosphatase to enhance catalytic rate constant.