Prof.Fumihito Miura

Issuing time:2021-09-17 13:22


Fumihito Miura


Associate Professor


Kyushu University Graduate School of Medical Sciences


100-word   biography:

Fumihito Miura was born   in Aomori prefecture, the most northern part of Honshu island, Japan, in   1974. After graduating from a local high school, he moved to Tokyo to study   chemistry at Tokyo University of Science and graduated in 1998. During the   undergraduation, he worked at a hospital as a part-time technician, and where   he became familiar with molecular biology. To study molecular biology   further, he moved to the Graduate School of Science, University of Tokyo. His   mentors were famous Japanese genome scientists Yoshiyuki Sakaki and Takashi   Ito. Since then, his focus has been on developing technologies for   genome-scale measurement.


Toward overlaying multiple epigenomes on   the methylome


Epigenomes form the   basis of gene expression. Nucleosome positioning, histone post-translational   modifications, transcription factor bindings, and DNA methylation have been   well documented for their strong association with gene expression. These   epigenetic modifications, except DNA methylation, are currently detected by   DNA fragmentation-based methods. The most common technique for identifying   the epigenetic state of chromatin is chromatin immunoprecipitation (ChIP). In   the ChIP assay, the chromatin is first fragmented, the target epigenome is   enriched with a specific antibody, and the collected DNA around the epigenome   is sequenced using Illumina's short-read sequencer.

Recently, DNA sequencing   technology has been advancing. Especially, the advent of palm-size sequencers   developed by Oxford nanopore technologies (ONT) is transforming the world of   DNA-sequencing-based techniques. The most distinct specificities of the   devices are long-read sequencing and direct detection of DNA modifications.   Therefore, to fully utilize the superior abilities of the new sequencer, the   epigenomes should be detected without DNA fragmentation. However, for the   realization of such measurement, a new principle should be devised.

To this end, we are   developing a new DNA methyltransferase-based epigenome quantitation   technology termed proximity DNA methylation (PDM). In PDM, DNA   methyltransferases are anchored on the chromatin via the specificities of   antibodies for the target epigenomes. Thus, the DNA methyltransferases would   introduce a methyl moiety to the DNA around the epigenomes, which enables the   detection of target epigenome in the form of DNA methylation. In this   presentation, I would like to show you the current state of the development.